Dr Mark Fowler
Undergraduate and postgraduate bioscientist / geneticist
SCOOTER106a_Lab technique ARMS (PDF)
SCOOTER106b_Lab technique ARMS (PPT)
SCOOTER108a_Lab technique RFLP (PDF)
SCOOTER108b_Lab technique RFLP (PPT)
ARMS (Amplification-refractory mutation system) is a PCR technique used in sickle cell disorder molecular diagnosis. (ARMS-PCR). These resources contain figures that step you through the basis of the process.
RFLP (Restriction fragment length polymorphisms) are other molecular techniques for identifying β-globin mutations in sickle cell disorder. A β–globin gene mutation is a common error which affects haemoglobin structure. You may wish to refer to our other genetics animated resources to explain these processes in more detail.