Dr Mark Fowler
Undergraduate bioscience student, post graduate bioscientist.
Video depicting ASO hybridisation (above)
SCOOTER107a_Lab technique ASO (PDF of slides)
SCOOTER107c_Lab technique ASO Hybridisation Transcript (PDF of video transcript and notes)
A resource describing ASO hybridisation which is a common tool in molecular biology, genetic testing or forensic research.
In the human disease sickle cell, there is a genetic mutation in the blood protein beta-haemoglobin. ASO can be used to detect the mutation in a DNA sample and therefore is used to identify the presence of the sickle cell mutation.