ASO Hybridisation technique for detecting gene mutations

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Content Author:
Dr Mark Fowler

Level:
Undergraduate bioscience student, post graduate bioscientist.

OER Features:
Video depicting ASO hybridisation (above)

SCOOTER107a_Lab technique ASO (PDF of slides)

SCOOTER107c_Lab technique ASO Hybridisation Transcript (PDF of video transcript and notes)

OER Description:

A resource describing ASO hybridisation which is a common tool in molecular biology, genetic testing or forensic research.

In the human disease sickle cell, there is a genetic mutation in the blood protein beta-haemoglobin. ASO can be used to detect the mutation in a DNA sample and therefore is used to identify the presence of the sickle cell mutation.